| Publication Type: | Journal Article |
| Year of Publication: | 2007 |
| Authors: | A. Ablordey, Fonteyne, P. - A., Stragier, P., Vandamme, P., Portaels, F. |
| Journal: | Clin Microbiol Infect |
| Volume: | 13 |
| Issue: | 7 |
| Pagination: | 734-6 |
| Date Published: | 2007 Jul |
| ISSN: | 1198-743X |
| Keywords: | Africa, Bacterial Typing Techniques, DNA, Bacterial, Humans, Minisatellite Repeats, Mycobacterium Infections, Nontuberculous, Mycobacterium ulcerans, Polymerase Chain Reaction, Sequence Alignment, Sequence Analysis, DNA, Species Specificity |
| Abstract: | Intra-species discrimination in the highly clonal pathogen Mycobacterium ulcerans was studied in a diverse collection of isolates by PCR amplification of a short sequence repeat locus containing heterogeneous arrays of tri-nucleotide repeats with an ACC consensus pattern. Post-amplification analysis indicated excellent typeability and identified five M. ulcerans alleles, including a unique Angolan type differentiated for the first time among a genetically conserved cluster of African isolates. These results are consistent with previously investigated independent markers, and provide an additional locus for variable number tandem repeat-based typing of M. ulcerans. |
| DOI: | 10.1111/j.1469-0691.2007.01716.x |
| Alternate Journal: | Clin. Microbiol. Infect. |